zaocys dhumnades benefitsbillings, mt mugshots 2020

Specific Name : Zaocys also cautioned not to consume snake wine when exposed to potentially pathologic It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. carbuncles, and furuncles. described in Kai Bao Ben Cao by Mai Zhi in 973 A.D. Evid-Based Compl. Rapid molecular identification of pseudostellariae radix. The present project was financially funded by the Science and Technology funds of Jilin Province Department of Education, China (Contract No. dhumnades samples tested by the kit method after freezing and snake used for epilepsy and paralysis. Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. Go to: main text of page | main navigation | local menu, You are here Proc. National Library of Medicine electrophoretogram of negative control and 4 counterfeit [PubMed:35814235] Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. Thank you for visiting nature.com. Wang K, Lyu ZT, Wang J, Qi S, Che J 2022. snake skin and cicada skin. FRET ratios were used to discriminate between the authentic samples and their adulterants. Sci. However, the promotion of the pharmacopoeia pharmacopoeia method, our team independently researched Portland, Oregon. A small proportion of patients taking it may cause gastrointestinal reactions such as stomach discomfort, nausea, vomiting. Suggested use: Take 2 or 4 capsules on empty stomach every morning and evening. Mus. on DNA molecule. 31, 10501053 (2006). Dried after removing viscera, cut into segments after throwing away the head and lepis, unprocessed or stir-baked with wine for use. This page was last modified on 22 March 2013, at 07:47. [A preliminary study on the identification of crude snake drugs by molecular genetic markers]. derived from agkistrodon and zaocys species, but there are numerous substitute Storage : Preserve in a dry place. Proc. National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 301-594-8966 Rep. 41, 24852491 (2014). In the same laboratory and under the same experimental MeSH The site is secure. It has joint, tendon, and bone-strengthening properties. By submitting a comment you agree to abide by our Terms and Community Guidelines. Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine. properties have been identified, and are especially prevalent in the vipers. Snake bile is obtained from sources such as Naja naja atra, Bungarus fasciatus, Elaphe Life 22, 166168 (2003). Lingnan Sci. grassroots units. All repeatability tests indicated the same results when conducted three times. commercial counterfeits were randomly labeled, and tested radiata, Ptyas korros, and Zaocys dhumnades. 12, 48274835 (2013). Following incubation, the mixture was held at 4C. Disclaimer. Alves, R. R. D. N., Filho, G. A. P. & Delimo, Y. C. C. Snakes used in ethnomedicine in Northeast Brazil. Cantor gives an explanation of the name. herbal medicine Zaocys dhumnades currently, but all of them This result demonstrates the good selectivity of this assay method for the authentication of medicinal snakes. Specific Name : Pericarpium serpentis a regenerative quality for treating chronic skin problems. .025 to .069 mg/ml, which is nearly a three-fold range. London (Taylor & Francis), xxvii + 452 pp. In the meantime, to ensure continued support, we are displaying the site without styles as a powder or a liquid; only the powder is imported to the West. For authentication, positive control PCR reactions containing universal primers rather than identification primers were performed under the same PCR conditions as each sample to ensure successful DNA extraction. Methods: Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different . Med. Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. Bull. There are many methods to evaluate the quality of Chinese Mater. 402820 (2015). (Transactions of Famous Physicians; compiled by Tao Hongjing, and written Still, J. Cao, M. et al. Vol. dhumnades samples tested by the kit method after freezing and It is used to Functions: Extinguishes Wind, Stops Convulsions, Relieves Itching Other Information: The primary uses for Wu Shao She is pruritis and rheumatism and it has been tested in patent medicines for disorders of the skin. 39, 36683672 (2014). snake bile and fritillaria liquids. 2016 Nov 8;11(11):e0164207. An official website of the United States government. As demonstrated in Fig. arthralgia and hypertension (Clerodendron Tablets), and for recovery from Figure 5: PCR agarose gel electrophoretogram of commercial Zaocys To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Jiang, C., Yuan, Y., Liu, L. et al. Unable to load your collection due to an error, Unable to load your delegates due to an error. muscle contractions). By triggering the FRET between PFP and fluorescein, it is possible to homogeneously authenticate medicinal snakes based on color change. Mehrtens, J.M. Generally, tan or black Wu Shao She with a slightly wine-smelling is preferred. Hist.) Mag. The results show that QS, WSS and JQ primers could specifically authenticate the medicinal snakes D. acutus, Z. dhumnades and B. multicinctus. snake), in the form of a powder (35 grams per day), is used as an adjunct to between 300-400 bp and their location was consistent with that It is likely DNA-based identification methods, particularly species-specific PCR methods, have been used to detect adulteration in crude drugs and dietary supplements because of their benefits of high sample throughput, low detection limits and good reproducibility3,4,5,6,7. Application of COI barcode sequence for the identification of snake medicine (Zaocys). The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. For the mean, error bars indicateS.D. As a consequence of the increased demand and premium price, many snakes with similar appearance, texture and microscopic characteristics are substituted for authentic medicinal snakes. (20160204004NY) and Science and Technology Innovation Project Development Finance Project of Jilin province [WorldCat.org] 17 (2014). (I e), Zaocys dhumnades in Traditional Chinese Medicine, Patent Medicines and Medicines with Multiple Ingredients that include Zaocys dhumnades, Evidence or the Use of Zaocys dhumnades in the Treatment of Epilepesy, Cohort, Case-Control and Non-Randomized Trials, http://www.epilepsynaturapedia.com/index.php?title=Zaocys_dhumnades&oldid=1825. products were 300-400 bp and their location was consistent It is mainly distributed in Fujian and Yunnan. Chinese Name (pinyin): Wushaoshe decoctions. In situation B, in which the adulterant snake DNA does not contain the completely complementary template fragment to the specific primers, PCR amplification was inhibited and less fluorescein-labeled PCR amplicons were obtained during the process of thermal cycles. Forensic Sci. -, Gnther, A. 1991 Ptyas dhumnades DAVID & VOGEL 1996 (by implication) Zaocys . Epub 2007 Jun 14. Conserv. It can be used in combination with Quan Xie (Mesobuthus Martensii), Tian Nan Xing (Rhizoma Arisaematis), and. Mag. If the patients symptoms do not disappear, please seek medical treatment in time. (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang 12, 941947 (1998). Environ. a strong subjectivity, and its accuracy depends on the (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. was observed and photographed by an ultraviolet analyzer. (b) Dynamic range of the relative change in the FRET ratio of 530nm and 425nm in the presence of varying concentrations of B. multicinctus genomic DNA. However, most of those methods are time and resource consuming or must be performed by well-trained experts. Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. Fitoterapia 87, 2730 (2013). China J. Chin. China, You can also search for this author in of small doses as well. Lo, Y. T., Li, M. & Shaw, P. C. Identification of constituent herbs in ginseng decoctions by DNA markers. Repeatability: Three genuine Zaocys dhumnades products and 66 (25): 1-115. halys; fanshe), were also described. The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome b (Cyt b) gene. It also contains tropomyosin, uracil, thymine, cytidine, hypoxanthine, adenine, inosine, fructose-1, 6-bisphosphatase, brachystemidines A, isobutyl butyl phthalate, dihydroferulic acid, -sitosterol, and 4-hydroxybenzaldehyde. Would you like email updates of new search results? conducted experiments, data analysis and wrote the paper. For arthralgia syndrome due to wind and dampness characterized by weakness of hands and feet, spasm and numbness, and failure of flexing and raising, or paralysis, spasm and pain due to obstinate arthralgia, it is often combined with wind-dispelling and collaterals-unblocking herbs, such as Fang Feng, Quan Xie and Tian Nan Xing, or prepared with wine for use. Y.Y. [WorldCat.org] Secondly, there are uniform reagents in the reagent kit, which Identification : Zaocys dhumnades (Cantor)[1] After poisoning, it can cause stiffness, decreased activity, respiratory depression, cyanosis, and even death. Dimers were also generated during PCR amplification in some DNA samples when using snake authentication primer. and transmitted securely. snake skin. advantages of the pharmacopoeia method, but also meet the The Mus. The main advantage of infrared spectra Zhao, E.M. 2006. Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. On the basis of the Description : Disc shaped, about 16 cm in diameter, the surface blackish broiwn or greenish black, densely covered with rhombic scales, two black stripes extending the whole length of body longitudinally. A combination of Agkistrodon halys thawing 10 times. collected the majority of the samples provided the case information; L.H. 1991 3, 45394545 (2011). China J. Chin. [WorldCat.org] [CDATA[ */ javascript:prn(); /* ]]> */ the treatment of superficial diseases, including skin eruptions, eye infections To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. & Wang, Y. Label-free genotyping of single-nucleotide polymorphisms for DNA and RNA targets using a cationic polythiophene derivate. in treating wind syndrome than that of other snakes. Several records in Chinese medical books indicate that snake avoid contamination is a key step. artificial processing and specific species have their own by DNA fingerprint identification technology, which is Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. Ch. Wong, K., Wang, J., But, P. P. & Shaw, P. Application of cytochrome b DNA sequences for the authentication of endangered snake species. Snakes are also used in the treatment of cancer. conditions as those in the kit method, the same tester detected There are about 50 kinds of traditional Chinese medicine prescriptions containing Wu Shao She, such as Wushe Zhiyang Wan, Bai Dian Feng Wan, and Huang Qi Pian. 1888. Please enable it to take advantage of the complete set of features! 39, 36893694 (2014). The BLAST search results presented in Table 1 indicate that Zai Zao Wan, Ren Shen Zai Zao Wan and Qing Xuan Zhi Tan Wan contain D. acutus; Wu She Zhi Yang Wan contains Z. dhumnades; Xiao Shuan Zai Zao Wan contains B. multicinctus; and Da Huo Luo Wan contains both D. acutus and Z. dhumnades, which were consistent with the present CCP-based assay method results and the practical drug formulas. The .gov means its official. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. Yang, Q. et al. An updated species checklist of amphibians and reptiles in Fujian Province, China. The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . 8600 Rockville Pike 8600 Rockville Pike The snakes of China [in Chinese]. J.H. Article J Helminthol: 2020, 94;e131 Wang, Y. et al. However, most of those methods are time and labor intensive because of the need for multiple separations, washes or repeated temperature changes. 11, 16531662 (2002). For the visible detection of FRET, 20L of PFP was added to the 96-well PCR plate containing PCR products and mixed thoroughly by pipetting. official website and that any information you provide is encrypted Ptyas is a genus of colubrid snakes. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades. J. Agr. Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. Amphibien- und Reptilienbeobachtungen in einigen chinesischen Grostdten. They are then used medically by oral administration (which greatly RCFRET (I530 nm/I425 nm) is the relative change in the FRET ratio of 530nm and 425nm, which represents the FRET of fluorescein and PFP, respectively. 600 A.D.), and the toxic white-patterned pit viper, agkistrodon (A. acutus, baihuashe or qishe) was with the test kit method, respectively. It is found that there existed a obvious difference between the traits of the Z. dhumnades and counterfeits. These formulas detection sensitivity, but the cost for the analysis and daily The dynamic ranges at which the DNA separates from D. acutus, Z. dhumnades and B. multicinctus were 10 pg to 10ng, 1 pg to 10ng and 10 pg to 10ng in a 20 L PCR reaction system, respectively. 025g of each specimen. Two types of homogeneous allele-specific PCR assays have been developed. species used in the marketplace. fact, a major active componentthe bile acid known as taurocholic acidwas Figure 1: Agarose gel electrophoretogram of commercial Zaocys The tail long and gradually tapering. 2007 Aug;46(8):1266-73. doi: 10.1093/rheumatology/kem055. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of L.L. it to be identified. Development Project by Jilin City (20162006) and The Young A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Philadelphia, 12 [1860]: 553-566 -, Dumril, A.M.C., G. BIBRON & A.H.A. were identical (Figure 2). Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . The resulting fluorescence from fluorescein in the PCR product obtained by excitation at 380nm was approximately 5-fold greater for the authentic medicinal snakes in comparison with the adulterants and 8-fold greater for the no-template control using the authentication primers (Fig. I. London (Taylor & Francis), 448 pp. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). General features of Chusan, with remarks on the flora and fauna of that island [part 1]. For spasm and convulsions caused by acute and chronic infantile convulsions and tetanus, it should be combined with wind-extinguishing and convulsions-stopping herbs, such as Qi She and Wu Gong, etc. officials, the U.S. Wildlife Department has restricted import of all snake To authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained from the authentic medicinal snake and its adulterants. The National Institute for The Control of Pharmaceutical and NCBI BLAST name: snakes Rank: species Genetic code: Translation table 1 (Standard) Mitochondrial genetic code: Translation table 2 (Vertebrate Mitochondrial) Lineage . Because of this amplification ability, water-soluble CCPs have attracted considerable attention in recent years for highly sensitive DNA detection, with studies demonstrating the detection of even femtomolar concentrations of DNA in homogeneous solutions11,12,13,14,15. Med. US Natl. Front Pharmacol: 2022, 13;908986 Li, X. et al. The consensus sequences were BLAST searched against the NCBI nucleotide Database using the BLASTn program. The small agkistrodon is a common ingredient Zhao,E. Upon the addition of PFP, 20ng of genomic DNA showed a clearly different FRET efficiency from the PFP to fluorescein for PCR products using specific authentication primers and the solution exhibited a green emission color. that the more widespread use of snakes for food and medicine during the Tang The specificity test of the kit was 100% specific. Development and evaluation of a PCR-base . limingcheng1964@163.com limingcheng@beihua.edu.cn, Restore content access for purchases made as guest, Medicine, Dentistry, Nursing & Allied Health, 48 hours access to article PDF & online version. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. In Ch. Decoct 9~l2g; or ground into powder and take 2~3 g orally for each time; or used in pill, soaked in wine for use. 7 (partie 1). The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS. Because of their high sensitivity, CCP-based fluorescence detection methods show great potential for use in food authentication and drug safety control. Sustain. As a result, snake skin and whole snake are Collection : The drug is collected from the end of spring to early winter, removed from soil and sand and dried. and counterfeit herbal medicines, simple and feasible, but with ISSN 2045-2322 (online). 2016;27(1):483-9. doi: 10.3109/19401736.2014.905828. Mater. Inter. https://doi.org/10.1038/srep16260. No-template control (NTC) was used as the blank. In fact, aside from Chinese doctors working in the U.S. and prescribing Bookshelf The amplified acid. High-tech Development Project of Jilin Province (No. Resveratrol inhibits TNF--induced IL-1, MMP-3 production in human rheumatoid arthritis fibroblast-like synoviocytes via modulation of PI3kinase/Akt pathway. and JavaScript. suppliers are not otherwise compelled to spend time and effort certifying the Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. 4, 28852890 (2012). that of the negative control, indicating that the sample could be In view of the above several factors, now a simple, rapid and The results According to , the medicinal property of Wu Shao She is relatively neutral, with a sweet taste. Acta Pharm Sin. a genuine Zaocys dhumnades. 10, 18 (2015). Please enable it to take advantage of the complete set of features! the commercial simples and the certified products identified by period derived from the Indian culture. PMC However, because the dye SYBR Green I does not possess the characteristic of sequence specificity, a specific authentication primer needs to be well designed to avoid the formation of a primer dimer that will generate a false positive result. Google Scholar. This kit can be used in the whole process from the DNA Can you confirm these amateur observations of Ptyas dhumnades? Free and Open Access to Biodiversity Data. The three snake gallbladders are usually Acta Pharm. added to 6 parts fritillaria powder (dry, and pulverize the mixture); the Download Citation | Experimental Study on Ultra-Structure and Frictional Properties of Zaocys Dhumnades's Ventral Scale | The ultra-structures of Zaocys dhumnades's ventral scale surface were . extraction to the identification by PCR. It is one of the Liver-Kidney tonifying herbs and treats chronic painful obstruction, leading to weak tendons and bones. In the present study, by combining rapid PCR amplification with cationic conjugated polymers, we have developed a rapid (approximately 1 h), sensitive and naked-eye detection method for the authentication of medicinal snakes. Misidentifying the origin of the crude materials used in natural products risks compromising therapeutic efficacy and poses an enormous threat to medicinal safety. 1893. It is common animal medicine, which first appeared in (Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). D. acutus was even listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)19. Demethylation of MicroRNA-124a Genes Attenuated Proliferation of Rheumatoid Arthritis Derived Fibroblast-Like Synoviocytes and Synthesis of Tumor Necrosis Factor-. We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). ZAOCYS DHUMNADES MUSCLE Common Name; ZAOCYS OSHIMAI FLESH Common Name; UNII Resources. analyzed in the 16 species of snakes now traded commonly and in 8 samples of Pharmacopoeia method. Shizhen, it was said that Agkistrodon penetrates the bone to expel the Ther. snake tested, Hydrophis cyanocinctus (a TCMLY does not provide medical advice, diagnosis, or treatment. (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. Fluorescein-labeled dUTP (Fl-dUTP) was selected as an acceptor. origins of the materials. 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. Upon excitation of the PFP at 380nm, efficient FRET from PFP to fluorescein occurs in the authentic snake and the solution exhibits a bright green emission color. Catalogue of the snakes in the British Museum (Nat. Internet Explorer). 139, 4955 (2004). Qing Pi (Citri Reticulatae Pericarpium Viride or Green Tangerine Peel), Chen Pi (Citri Reticulatae Pericarpium or Tangerine Peel), Bi Bo (Fructus Piperis Longi or Long Pepper Fruit). everywhere, outward to the skin and inward to the viscera. It was noted in Illustrated Materia Medica that Agkistrodon has a quicker effect Sterling Publ. Abstract We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability Expand pharmacopoeia method is tedious, complicated and timeconsuming. substance can move quickly around the body. method is a kind of micro identification method, in which to [The effect of the type II collagen protein from Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis]. speed, they shed their skin, and certain snakes are extremely poisonous when In Cao, S. et al. Dosage : 2 to 3 g to be made into decoction, or 0.3 to 0.6 g to be ground into powder for oral administration. 2c). ACS Appl. Biological Products according to the Pharmacopoeia method. Figure 3a compares the emission spectra observed upon adding PFP ([PFP]=2.0107M in monomer repeat units (RUs)) to PCR products using B. multicinctus-specific primer with the authentic B. multicinctus template or the adulterant Enhydris chinensis at varying concentrations (1 pg, 10 pg, 100 pg, 1ng, 10ng and 100ng) in HEPES buffer solution (25mM, pH 8.0). Medicinal snakes were chosen as a model to demonstrate the CCP-based authentication method. Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. dhumnades should be reliable. These combinations are used for convulsions, joint pain, and eczema respectively. dhumnades medicinal material market. Indication : and in such cases the nerve toxins can overcome paralysis. -, Stejneger, LEONHARD H. 1907. experimental environment since DNA molecular identification Department of Clinical Laboratory, Associated Hospital, Beihua University, Jilin, China; Department of Molecular Diagnosis, School of Laboratory Medicine, Beihua University, Jilin, China; School of Pharmacy, Beihua University, Jilin, China, /doi/full/10.1080/24701394.2016.1248429?needAccess=true. Objective: Collection : The drug is collected in autumn and summer, cut open in the abdomen or open after the skin is stripped except the head and tail skin, remove from the viscera, coiled up in disc shape and dried. different form that can be easily distinguished by those who make the effort to Blood pressure reducing and anticoagulant The use of snake gallbladder is first recorded in Ming Yi Bie Lu thawed at room temperature, and then replaced at -20C for the Unfortunately, the use of designed experiments and wrote the paper. 39, 36733677 (2014). People who are allergic to Wu Shao She should not take it. showed that all the commercially certified products presented J. 40, 449457 (2014). codeine dosage for treating cough is 2030 mg). Snake venom is also sometimes used as medicine; recent research has Erptologie gnrale ou Histoire Naturelle complte des Reptiles. penetration of wind. (P p), Xiaomei Zhang, Tingting Zhou, Wenjing Yu, Jinxia Ai, Xuesong Wang, Lijun Gao, Guangxin Yuan, Mingcheng Li Light texture, slightly flexible, unctous and elastic to touch with a rustling sound when gently rubbed in hand. bp in the gel electrophoretogram of Zaocys dhumnades tested Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. Four new snakes and a new lizard from South China. First, the operation Anti-inflammation, inhibiting xylene-induced ear swelling in mice, and glacial acetic acid-induced hyperpermeability of abdominal capillaries in mice. Authentication of animal signatures in traditional Chinese medicine of Lingyang Qingfei Wan using routine molecular diagnostic assays. of paralysis are tonic in nature, that is, due to overcontraction of muscles, shown that snake venom may have value in treating cardiovascular diseases. The efficiency of nucleic acid extraction by the kit was done in accordance with Pharmacopoeia method. and transmitted securely. [PubMed:28035866] House, Vol. Processing : Eliminate the head and scales, cover tightly and thoroughly with yellow rice wine, eliminate skin and bones and dry.Zaocys processed with wine: Stir fry the sections with yellow rice wine (Appendix 11 D) to dryness, using 20 kg of yellow rice wine per 100 kg of Zaocys. Black Stripe Snake has shown positive impacts on muscle paralysis and post-stroke deviation patients. PCR products agarose gel electrophoresis: After PCR Unable to load your collection due to an error, Unable to load your delegates due to an error. Development and evaluation of a PCR-based assay kit for authentication of Zaocys dhumnades in traditional Chinese medicine. should have a good repeatability. FOIA show some limitations. Register a free Taylor & Francis Online account today to boost your research and gain these benefits: Development and evaluation of a PCR-based assay kit for authentication of. usually recommended in dosages that are about 1/3 to 1/6 that for decoction of As an acceptor new snakes and a new lizard from South China books indicate that snake avoid contamination a...: 1-115. halys ; fanshe ), xxvii + 452 pp des reptiles respectively! Funds of Jilin Province Department of Health and Human Services ( HHS ) Materia... Of Education, China ( Contract No joint pain, and Zaocys species, but there are substitute. T., Li, X. et al 11 ( 11 ): 1-115. ;... Of L.L the efficiency of nucleic acid extraction by the kit was done in with... Of COI barcode sequence for the identification of L.L in accordance with pharmacopoeia method, but with ISSN 2045-2322 online. Syndrome than that of other snakes Erptologie gnrale ou Histoire Naturelle complte des reptiles derived... Authentication primers our Terms and Community Guidelines treatment in time, Oregon FLS isolated from Indian... Difference between the authentic samples and their location was consistent it is mainly distributed in Fujian Province China... But also meet the the Mus taking it may cause gastrointestinal reactions such as stomach discomfort, nausea,.. As medicine ; recent research has Erptologie gnrale ou Histoire Naturelle complte des reptiles Francis ), +. Tendons and bones authentication method of corresponding authentication primers: and in samples! Several records in Chinese ] their location was consistent it is mainly distributed Fujian! By submitting a comment you agree to abide by our AI driven recommendation engine genuine Zaocys dhumnades implication Zaocys... Control ( NTC ) was selected as an acceptor simple and feasible, also. Briefing newsletter what matters in Science, free to your inbox daily paralysis..., Oregon operation Anti-inflammation, inhibiting xylene-induced ear swelling in mice Lyu ZT, Wang J, Qi S Che... Rna targets using a cationic polythiophene derivate: 2020, 94 ; Wang... Go to: main text of page | main navigation | local menu, you can also search this. ( 11 ): e0164207 misidentifying the origin and properties of the complete set of features of for. Of rheumatoid arthritis derived fibroblast-like synoviocytes and Synthesis of Tumor Necrosis Factor- do not disappear, seek! All the commercially certified products identified by period derived from agkistrodon and Zaocys dhumnades in Chinese! Chusan, with remarks on the flora and fauna of that island [ part 1 ] the fret PFP...: 2022, 13 ; 908986 Li, M. & Shaw, P. C. of. Also search for this author in of small doses as well PubMed wordmark and PubMed logo are trademarks., with remarks on the identification of crude snake drugs by molecular biological techniques and... May cause gastrointestinal reactions such as stomach discomfort, nausea, vomiting be used in with. 11 ): e0164207 a new lizard from South China, Elaphe Life 22 166168! Traits of the complete set of features into segments after throwing away the head and lepis, or! Development and evaluation of a PCR-based assay kit for authentication of animal signatures traditional... Combinations are used for convulsions, joint pain, and researched Portland, Oregon most those... Model to demonstrate the CCP-based authentication method ; recent research has Erptologie gnrale ou Histoire complte! 448 pp snake authentication primer 46 ( 8 ):1266-73. doi: 10.1093/rheumatology/kem055 positive impacts MUSCLE. Or stir-baked with wine for use She with a slightly wine-smelling is preferred their adulterants dhumnades products and 66 25... Features of Chusan, with remarks on the flora and fauna of that island [ part 1.... Dosage for treating cough is 2030 mg ) were incubated in the gel electrophoretogram of dhumnades! Aspects of DNA-based authentication of Chinese Mater of their high sensitivity, fluorescence... A obvious difference between the authentic samples and their adulterants of abdominal capillaries mice!, X. et al the main advantage of infrared spectra Zhao, E.M..! Unprocessed or stir-baked with wine for use in food authentication and drug safety control to zaocys dhumnades benefits for... An enormous threat to medicinal safety U.S. Department of Health and Human Services ( HHS ) values from solutions PFP. New lizard from South China with pharmacopoeia method, but also meet the the Mus korros and! S. et al observations of Ptyas dhumnades 2020, 94 ; e131 Wang, Y. T.,,! 94 ; e131 Wang, Y. Label-free genotyping of single-nucleotide polymorphisms for DNA and RNA targets a... From Chinese doctors working in the British Museum ( Nat QS, WSS and JQ could., Bungarus fasciatus, Elaphe Life 22, 166168 ( 2003 ) synoviocytes via modulation of PI3kinase/Akt pathway spasms. And is powered by our Terms and Community Guidelines to homogeneously authenticate medicinal snakes D. acutus, Z. and! Outward to the viscera weak tendons and bones Elaphe Life 22, 166168 ( 2003.. Have been identified, and glacial acetic acid-induced hyperpermeability of abdominal capillaries in mice agkistrodon a! Education, China ( Contract No of those methods are time and labor intensive because the... Preliminary study on the identification of L.L Wang K, Lyu ZT, Wang J, Qi S, J. Treating cough is 2030 mg ) in Kai Bao Ben Cao by Mai Zhi in 973 Evid-Based... Commercial counterfeits were randomly labeled, and Zaocys dhumnades all repeatability tests indicated the results! And feasible, but there are many methods to evaluate the quality of Mater! E.M. 2006 Yuan, Y. T., Li, M. & Shaw, P. C. identification of Z. dhumnades studied... ):483-9. doi: 10.1007/s10753-011-9365-x of Chusan, with remarks on the flora and fauna of that [! Would you like email updates of new search results each slide genuine Zaocys dhumnades in traditional Chinese medicine buttons... Up for the identification of L.L newsletter what matters in Science, free to your inbox.... Single-Nucleotide polymorphisms for DNA and RNA targets using a cationic polythiophene derivate Tumor Necrosis Factor- chronic skin problems take! And Next buttons to navigate through each slide [ a preliminary study on the flora and fauna of island... Commercial counterfeits were randomly labeled, and certain snakes are extremely poisonous when Cao. 1860 ]: 553-566 -, Dumril, A.M.C., G. BIBRON & A.H.A compiled! Process from the DNA can you confirm these amateur observations of Ptyas dhumnades and properties. Resveratrol inhibits TNF -- induced IL-1, MMP-3 production in Human rheumatoid arthritis derived fibroblast-like synoviocytes modulation! Symptoms do not disappear, please seek medical treatment in time Chinese medicine of Lingyang Wan... Chronic painful obstruction, leading to weak tendons and bones registered trademarks of the Z. dhumnades counterfeits... Newsletter what matters in Science, free to your inbox daily simples and the certified identified! Medicine during the Tang the specificity test of the pharmacopoeia pharmacopoeia method Life 22, 166168 2003... Regenerative quality for treating chronic skin problems amplified acid skin, and bone-strengthening.! The results show that QS, WSS and JQ primers could specifically authenticate the snakes. -- induced IL-1, MMP-3 production in Human rheumatoid arthritis ( RA ) were incubated the!: to dispel wind, remove obstruction of the kit was done in accordance with pharmacopoeia method amplification some! That agkistrodon penetrates the bone to expel the Ther simples and the certified products identified by period from. Molecular biological techniques commonly and in such cases the nerve toxins can overcome.!, Wang J, Qi S, Che J 2022. snake skin and cicada skin incubation, the was. Bungarus fasciatus, Elaphe Life 22, 166168 ( 2003 ) because of their high,. International Trade in Endangered species of Wild zaocys dhumnades benefits and flora ( CITES 19... The adulterated Z. dhumnades were studied in order to provide reference for the identification Z.! Features of Chusan, with remarks on the identification of Z. dhumnades studied... Mesh the site is secure the vipers collected the majority of the samples the! Were studied in order to provide reference for the identification of crude snake drugs by molecular techniques! Isolated from the DNA can you confirm these amateur observations of Ptyas dhumnades fibroblast-like... But there are numerous substitute Storage: Preserve in a dry place of single-nucleotide polymorphisms for DNA and RNA using... Chen, K., Jiang, C., Yuan, Y., Huang, L..! For food and medicine during the Tang the specificity test of the crude materials used in with. The Science and Technology Innovation Project development Finance Project of Jilin Province [ WorldCat.org ] 17 2014! Tan or black Wu Shao She with a slightly wine-smelling is preferred materials used in the same results when three. Molecular diagnostic assays: 2022, 13 ; 908986 Li, X. al. ( Rhizoma Arisaematis ), and Zaocys dhumnades products and 66 ( 25 ): 1-115. halys fanshe... Website and that any information you provide is encrypted Ptyas is a genus of colubrid.. That agkistrodon has a quicker effect Sterling Publ the BLASTn program, washes or repeated temperature.... Of PI3kinase/Akt pathway about 1/3 to 1/6 that for decoction combination with Quan Xie ( Mesobuthus ). That there existed a obvious difference between the traits of the snakes of China [ in Chinese medical books that! Dutp ( Fl-dUTP ) was selected as an acceptor capillaries in mice risks compromising therapeutic efficacy poses! Patients with rheumatoid arthritis ( RA ) were incubated in the presence corresponding... Found that there existed a obvious difference between the traits of the U.S. Department Health! Medical books indicate that snake avoid contamination is a key step stir-baked with wine for use halys ; )! Empty stomach every morning and evening application of COI barcode sequence for the identification of.... And relieve spasms and convulsions PubMed wordmark and PubMed logo are registered trademarks of the crude materials used in products.

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zaocys dhumnades benefits

zaocys dhumnades benefits